About the laboratory
The Laboratory of Biochemical Research Methods (LBRM) was founded in 1975 on the basis of the Department of Biochemistry and Pathochemistry of Carbohydrates of the Central Research Laboratory and was a structural unit of the Research Division of Belarusian State Medical University (BSMU). Since 07/01/2025, LBRM became part of the Scientific Research Institute of Experimental and Clinical Medicine (SRIECM) as a result of the merger of the research division and the research laboratory within SRIECM.
The laboratory conducts research in the field of psychiatry, neonatology, surgery, obstetrics and gynecology, regenerative medicine, combustiology, and infectious diseases.
Recently, unique and high-tech techniques have been developed on the basis of LBRM:
1. for replacing defects in the articular surface of cartilage with differentiated stem cells;
2.for preparing allogeneic parathyrocytes for allogeneic transplantation in hypoparathyroidism;
3. for obtaining a biologically active biodegradable carrier for mesenchymal stem cell transplantation in case of adhesive disease;
4. for obtaining a graft based on mesenchymal stem cells, alginate hydrogel and porous pectin films used to prevent adhesions;
5. for topical treatment of diabetic skin ulcers in patients with diabetes mellitus using stem cells.
The authors were awarded the State Prize of the Republic of Belarus for the development of a technique for xenotransplantation of islet cells into the recipient's vascular bed.
Over the past 10 years, the LBRM staff has prepared 6 doctoral and 38 PhD dissertations for the defense of scientific assignments and joint projects with the departments of BSMU. Ove the same period, about 200 articles and more than 60 abstracts in home and foreign press have been published. 17 instructions for use, 7 patents and 5 laboratory regulations have been prepared and approved.
Main areas of activity:
1. Development of methods of hematological, immunological, genetic and biochemical analysis in areas that study the functioning of the body's metabolic systems in various pathological conditions in order to create new and improve previously existing methods and means of diagnosis, therapy, as well as medical prevention of diseases within the framework of state scientific and technical programs, individual scientific and technical projects, fundamental and exploratory research, agreements with ministries, organizations and institutions of all forms of ownership in the Republic of Belarus and abroad.
2. Study of the conditions of isolation and cultivation in vitro, phenotypic characteristics, ability to reproduce and differentiate human and animal mesenchymal stem cells in various directions.
Aims and objectives:
1. Development of new methods of medical prevention, diagnosis and treatment of human diseases.
2. Implementation of research results in clinical practice and the educational process.
3. Training of highly qualified scientific personnel (doctors and PhDs).
4. Advisory and methodological assistance to practical healthcare.
Hardware base:
The laboratory has all the necessary equipment to ensure scientific research. This equipment is used to carry out research both for performing scientific tasks at LBRM and for other laboratories and departments of BSMU, including dissertations by graduate students and doctoral students of BSMU.
Basic research hardware
- Heraeus laminar flow cabinets and CO2 incubators
- Multi-function spectrophotometer for microplates and cuvettes MultiskanTM SkyHigH (Thermo Fisher Scientific company, Finland)
- FC2060 Flow Cytofluorimeter (Perlong Medical Equipment Co., Ltd.)
- Clima MC-15 Semi-automatic Biochemical Analyzer
- Leica DM4000B Microscopes
- Olympus IX51 Inverted Microscope
- DT-322 detection amplifier
- Palm-Cycler Gradient Thermal Cycler
- Bio-Rad Gel Doc XR Gel documentation system
Research methods:
1. General clinical research
2. Hematological studies
3. Biochemical research
4. Determination of antioxidant status
5. Immunological research
6. Cytological studies
7. Obtaining cellular material for the cultivation of various types of cells
8. Preparation for cell culture research and transplantation
9. Obtaining, maintaining and testing transplanted cell cultures
10. Cryopreservation of cells and tissues
For research in the field of molecular biology, the polymorphism of genes is analyzed by polymerase chain reaction (PCR), followed by restriction analysis specific to each of the studied gene polymorphisms, and gel documentation.
The Leica DM4000B and inverted Olympus IX51 microscopes are used to study cell cultures and intracellular processes. Microscopes allow for fluorescence studies and are equipped with highly sensitive digital cameras.
The improvement of scientific and methodological support for research at the laboratory is carried out in the following main areas:
1. Development and verification of standardized experimental models of pathological conditions. The regulations of biological models that have been tested for many years and approved by international expert commissions have been chosen as standards.;
2. Introduction of modern research methods into the practice of medical and biological experiment;
3. Use of modern approaches to information support of research and development.
Group
Head of the laboratory – Ibragimova Zhanna Arkadyevna, PhD in Biological Sciences
- Antonina Viktorovna Goncharik, Senior Researcher
- Lyudmila V. Kartun, Senior Researcher
- Kolesnikova Tatiana Sergeevna, Senior Researcher
- Khodosovskaya Elena Vyacheslavovna, Senior Researcher
- Goranov Vitaly Anatolyevich, Leading Researcher, PhD
- Anastasia Kadirovna Abdul, Researcher
- Dudkina Lilia Garyevna, Research Associate
- Polina Igorevna Chepik, intern of a junior researcher
MAIN SCIENTIFIC ACHIEVEMENTS OF LBMI
for the period of 2021-2025
- The method for obtaining parathyrocyte cultures has been developed and the method for allogeneic transplantation of a biomedical cell product based on parathyrocytes and multipotent mesenchymal stromal cells in hypoparathyroidism has been approved;
- The technology of in vitro cell graft preparation to replace defects in the articular surface of cartilage has been introduced;
- A highly effective method of cell therapy of superficial skin ulcers in diabetes mellitus has been developed and experimentally substantiated;
- A transplant based on multipotent mesenchymal stromal cells, alginate hydrogel and porous pectin films has been obtained to prevent adhesions;
- Based on the comprehensive study of genetic and neurobiological fundamentals of mental disorders, the method for identification of molecular biological predictors of suicidal behavior has been developed;
- Immunological markers that characterize the severity of chronic obstructive pulmonary disease have been developed;
- Genetic and immunological criteria to evaluate the effectiveness of therapy for severe burn injury with high diagnostic significance and outcome prediction have been proposed.
Instructions for use
1. "A method for assessing the likelihood of intentional self-harm" (authors: Davidovsky S.V., PhD; Skugarevskaya M.M., DrSc, Professor; Ibragimova Zh.A., PhD; Marchuk S.I., PhD; Goncharik A.V., PhD; Babenko A.S., PhD; Khodzhaev A.V.), approved by the Ministry of Health of the Republic of Belarus 05.11.2021, No. 057-0621.
2. "A method for assessing the likelihood of mental and behavioral disorders associated with the postpartum period" (authors: Davidovsky S.V., PhD; Khvostova I.I., PhD, Associate Professor; Ibragimova Zh.A., PhD; Goncharik A.V., PhD; Kartun L.V., PhD; Semerikhina S.E., PhD), approved by the Ministry of Health of the Republic of Belarus No. 178-1221 dated December 23, 2021.
3. "A method for determining the probability of intentional self–harm in persons in a state of adaptation disorder" (authors: Davidovsky S.V., PhD; Ibragimova, Zh.A., PhD; Kostyuk S.A., DrSc, Prof.; Goncharik A.V.; Kartun L.V.; Semerikhina S.E., PhD; Khodzhaev A.V., PhD; Glinkina T.V.), approved by the Ministry of Health of the Republic of Belarus No. 140/1122 dated 11/30/2022.
4. "A method for determining the likelihood of mental and behavioral disorders during the period of adaptation to military service" (authors: Davidovsky S.V., PhD, Associate Professor; Skugarevskaya M.M., DrSc, Professor; Ibragimova Zh.A., PhD; Khodzhaev A.V., PhD; Kolesnikova T.S., Khodosovskaya E.V.), approved by the Ministry OF Health of the Republic of Belarus No. 150-1223 dated 06/24/2024).
5. "A method for identifying clinical and laboratory criteria for the risk stratification of thrombosis in patients with COVID-19, (authors: Khryshchanovich V.Ya., DrSc, Professor; Rogovoy N.A., PhD, Associate Professor; Skobeleva N.Ya., PhD; Goncharik A.V., Kartun L.V., Khodosovskaya E.V., Shustova D.A., Abiralo O.S., Register. No. 156-1224).
6. "A method for predicting the development of disseminated intravascular coagulation syndrome in patients with burn disease" (authors: Skakun P.V., MD; Alekseev S.A., DrSc, Professor; Ibragimova Zh.A., PhD; Krasko O.V., PhD, Associate Professor), Register. No. 113-1124.
Patents
1. Eurasian application for the invention "A method for predicting the risk of suicide by a male person in a state of suicidal crisis. Developers: Davidovsky S.V., Ibragimova Zh.A., Semerikhina S.E., Goncharik A.V., Kolesnikova T.S.). No. 2021/EA/0039 dated 06/24/2021, Patent issue date – 01/31/2023, No. 042278 B1
2. Eurasian application for the invention of a method for predicting the likelihood of suicide in women who are in a state of adjustment disorder. Developers: Davidovsky S.V., Ibragimova Zh.A., Kolesnikova T.S., application date 18.09.2023, No. 2023/EA/0053
3. Eurasian patent application for the invention "Method for the diagnosis of disseminated intravascular coagulation syndrome in patients with burn disease" Developers: Skakun P.V., Zhilinsky E.V., Alekseev S.A., Ibragimova Zh.A. dated 04/29/2024, No. 2024/EA/0026.