The Laboratory of Molecular Genetic Diagnostics was established in July 2025 as a result of the reorganization of the research laboratory. The laboratory traces its history back to 2001, when a new division, the PCR diagnostics group, was organized in the Research Laboratory of the State Educational Institution "Belarusian Medical Academy of Postgraduate Education" (hereinafter - the BelMAPE Research Institute). The main area of scientific activity of the PCR diagnostics group was the study of molecular genetic factors of the development of various pathological conditions of infectious and non-infectious origin. This scientific area was headed by S.A. Kostyuk, who successfully defended her doctoral thesis in 2010 on the issues of clinical laboratory diagnostics of sexually transmitted infections associated with HIV. Under the guidance of Kostyuk S.A., the laboratory staff of Rudenkov T.V. (2017), Poluyan O.S. (2019) and Glinkina T.V. (2024) successfully defended their PhD theses.
Laboratory staff
Poluyan Olga Sergeevna – Leading Researcher, Candidate of Biological Sciences, Associate Professor
Rudenkova Tatiana Vladimirovna – Leading Researcher, Candidate of Biological Sciences, Associate Professor
Lyamtseva Anna Konstantinovna – Junior Researcher
Marina Aleksandrovna Malashenko - laboratory assistant
Methods and techniques of clinical and experimental research:
- Qualitative analysis of DNA in biological material by PCR with electrophoretic detection scheme;
- Qualitative and/or quantitative analysis of DNA in biological material by real-time PCR;
- Qualitative and/or quantitative analysis of gene expression levels by reverse transcription PCR;
- Identification of polymorphisms in genes;
- Determination of nucleotide sequences in genes by sequencing PCR.
The main directions of scientific research activity:
1. Development and introduction into medical practice of new effective and more informative laboratory tests based on the assessment of the individual genetic profile of the patient, analysis of the molecular and genetic characteristics of the human microbiome.
2. Establishment of the molecular and genetic foundations of the etiology and pathogenesis of multifactorial diseases.
3. Development of molecular biological technologies for analyzing the structural features and functional state of genes.
4. Studies of the molecular and biological characteristics of infectious agents.
Main scientific results
Monographs:
1. Kostyuk, S.A., Kulaga, O.K. Associated urogenital infections in obstetrics and gynecology: a modern view of the problem. Monograph / O.K. Kulaga, S.A. Kostyuk. Minsk: Unipol, 2008. 540c.
2. Reproductive health of women with STIs / S.I. Mikhalevich, S.A. Kostyuk, M.N. Ismail. Minsk: Zmitser Kolas, 2008. 360c.
3. Kostyuk S.A. Clinical laboratory diagnostics of associated infections of the urogenital tract: monograph / S.A. Kostyuk. Minsk: BelMAPO Publ., 2012. 306 p.Molecular biological methods in medicine: a monograph / S.A. Kostyuk. Minsk: BelMAPO Publ., 2013. 327 p.
4. Kostyuk S.A., Kolomiets N.D., Rudenkova T.V., Poluyan O.S. Theoretical and applied issues of the application of nucleic acid analysis methods. Minsk: BelMAPO, 2014, 257 p.
5. Demidchik, Yu.E., Kostyuk S.A., Tretyak I.Y. Mechanisms of cellular chemoresistance in breast cancer / Yu.E. Demidchik, S.A.Kostyuk, I.Y. Tretyak. Minsk : Belorusskaya Nauka Publ., 2016. 152 p.
6. Skin atrophy : a monograph / I.G. Shimanskaya, N.V. Klimenkova, O.V. Pankratov, S.A. Kostyuk, T.A. Beach, V.V. Krumkachev, R.Y. Shikalov, T.V. Rudenkova, N.I. Kruk, V.A. Timoshenko, A.A. Khartonic – Minsk.: BelMAPO, 2018. – 302 p.
7. Microflora of the oral cavity and dental diseases / S.V. Zhavoronok, T.L. Shevela, S.A. Kostyuk. – LAP LAMBER, Internatoin book market Service Ltd, 2018. 119 p.
8. Microbial biofilms in periodontology and endodontics / Yudina N.A., Kostyuk S.A., Pivankova N.N., Yakovleva-Malykh M.O. – Minsk. : BelMAPO, 2020– 208 p.
9. Pathogenic properties of Mycoplasma pneumonia and Mycoplasma genitalium, optimization of their etiological diagnosis / S.A. Kostyuk, T.V. Rudenkova, T.V. Glinkina. Minsk : BSMU, 2024. 172 p. ISBN 978-985-21-1525-4.
Patents:
1. Method for determining the resistance of the causative agent of urogenital infection Chlamydia trachomatis, Mycoplasma hominis and Ureaplasma urealyticum to a natural or semi-synthetic tetracycline antibiotic: patent 8624 Rep. Belarus, IPK7 From 12 Q 1/04 G 01 N 33/50 / G. Y. Hulup, S. A. Kostyuk ; applicant GUO BelMAPO – No. a 20030986 ; application 29.10.2003; publ. 30.06.05 / / official bulletin / National Center intellectual. ownership. - 2006. - No. 2. - p. 174.
2. Method for determining the pathogens of urogenital infections Chlamydia trachomatis, Mycoplasma hominis and Ureaplasma urealyticum in the studied material: patent 7967 Rep. Belarus, IPK7 From 12 Q 1/04 G 01 N 33/50 / G. Y. Hulup, S. A. Kostyuk ; applicant GUO BelMAPO – No. a 20030985 ; application 29.10.2003; publ. 30.06.05 / / official bulletin / National Center intellectual. ownership. - 2006. - No. 2. - p. 174.
3. Method for determining the pathogens of urogenital infections Chlamydia trachomatis, Mycoplasma hominis and Ureaplasma urealyticum in the studied material : patent 8164 Rep. Belarus, IPK7 From 12 Q 1/04 G 01 N 33/50 / S.A. Kostyuk, G.Ya. Hulup ; applicant GUO BelMAPO – no. a 20030984 ; application 29.10.2003 ; published 30.06.05 // Afitsyny bul. / National Center for Electrical Engineering. ulasnasci. - 2006. – No. 2. – p. 173.
4. A method for determining the resistance of the causative agent of urogenital infection of the causative agent of urogenital infection Chlamydia trachomatis, Mycoplasma hominis and Ureaplasma urealyticum to a natural or semi-synthetic 14- or 15-membered antibiotic of the macrolide group: patent 8646 Rep. Belarus, IPK7 From 12 Q 1/04 G 01 N 33/50 / S.A. Kostyuk, G.Ya. Hulup ; applicant GUO BelMAPO – no. a 20030987 ; application 29.10.2003 ; published 30.12.06 // Afitsyny bul. / National Center for Electrical Engineering. ulasnasci. - 2006. – No. 2. – p. 173.
5. A method for detecting intrauterine infections of chlamydia, mycoplasma, or trichomonas etiology in a newborn: patent 10314 Rep. Belarus, IPK7 G 01 N 33/50 / G. Y. Hulup, N.A. Badygina, S.A. Kostyuk, M. N.Ismail ; applicant GUO BelMAPO – no. a 20050545 ; application 02.06.2005; published 28.02.08 / / official bulletin / National Center intellectual. ownership. - 2008. - No. 1. - p. 171.
6. Method for determining the DNA concentration of Mycoplasma hominis and Ureaplasma urealyticum in epithelial cells : Patent 10771 Rep. Belarus, IPK7 G 01 N 33/50 / Badygina N.A., G. Ya. Hulup, S. A. Kostyuk ; applicant GUO BelMAPO – no. a 20050482 ; application 18.05.2005; published 30.06.08 / / official bulletin / National Center intellectual. ownership. - 2008. - No. 1. - p. 172.
7. Method of drug treatment of cervical erosion caused by mixed pathogenic flora: pat. No. 10082 Rep. Belarus, IPC G 01 N 33/569/ O. K. Kulaga, S. A. Kostyuk, D. V. Buryak; applicants: O. K. Kulaga, S. A. Kostyuk, D. V. Buryak - No. a 20060079 ; application. 30.01.06; publ. 30.10.07 / / official bulletin / National The Intellectual Center. Ownership. – 2007. - № 6 (59). - P. 149.
8. Method of treatment of urogenital infection caused by association of protozoa, bacteria and viruses : pat. No. 11080 Rep. Belarus, IPC a 61K 31/04, a 61k 31/7088 / O. K. Kulaga, S. A. Kostyuk ; applicants : O. K. Kulaga, S. A. Kostyuk - No. a 20060869 ; application 30.08.06; publ. 30.08.08 / / official bulletin / National The Intellectual Center. Ownership. – 2008. - № 4.
9. Method of DNA isolation of Ureaplasma urealyticum or Mycoplasma hominis: Patent 11321 Rep. Belarus, IPC from 07H 21/00 / N.A. Badygina, S. A. Kostyuk, N.N. Poleshchuk, L. V. Rubanik ; applicant GUO BelMAPO – No. a 20060462 ; application dated 05/16/2006; published on 12/30/08 / / official bulletin / National Center intellectual. ownership. - 2009. - No. 1. - p. 172.
10. Method of creating the DNA calibrator of Ureaplasma urealyticum or Mycoplasma hominis: Patent 11635 Rep. Belarus, IPK7 G 01 N 33/50 / Hulup G. E., Badygina N.A., Kostyuk S. A., Kustanovich A.M. applicant GUO BelMAPO – no. a 20060463 ; application 16.05.2006; published 28.02.09 / / official bulletin / National Center intellectual. ownership. - 2009. - No. 1. - p. 173.
11.Method of diagnosis of juvenile periodontitis: pat. 14148 Rep. Belarus, IPC (2009) G 01 N 33/48 / Yudina N.A., Kostyuk S. A., Lugovskaya A.V., Glinkina T. V., Poluyan O. S., Rudenkova T. V. applicant GUO BelMAPO – no. a 20081344 ; application no. 24.10.2008; published 07.12.10 / / official bulletin / National Center an intellectual. ownership. - 2010. - No. 1. - p. 122.
12.Method of diagnosis of acute ulcerative necrotizing gingivitis: patent 14153 Rep. Belarus, IPC (2009) G 01 N 33/48 / Yudina N.A., Kostyuk S. A., Lugovskaya A.V., Glinkina T. V., Poluyan O. S., Rudenkova T. V. applicant GUO BelMAPO – No. a 20081378 ; application 31.10.2008; published 30.06.10 / / official bulletin / National Center an intellectual. ownership. - 2010. - No. 1. - p. 113.
13. "Method for obtaining a nutrient medium for the cultivation and detection of Mycoplasma genitalium" patent application for invention No. a20131298 dated 05.11.13. Authors: Rudenkova T.V., Kostyuk S.A., Rubanik L.V.
14. "Method for determining the expression of the GSTM1 gene in samples from paraffin-fixed sections of breast tumor tissue" application for obtaining a patent for invention No. 201500680/26 dated 16/25/15. Authors: Tretyak I.Yu., Demidchik Yu.E., Kostyuk S.A., Rudenkova T.V., Poluyan O.S.
15. A method for modeling Mycoplasma pneumoniae infection in vitro : pat. BY 23685 / T. V. Glinkina, S. A. Kostyuk, T. V. Rudenkova, O. S. Poluyan. – Published on 30.04.2022.
Provision of paid services to the public
The laboratory provides paid medical services to the public to identify pathogens of sexually transmitted infections
- Chlamydia trachomatis,
- Mycoplasma hominis,
- Mycoplasma genitalia,
- ureaplasma urealyticum,
- Gardnerella vaginalis,
- Neisseria gonorrhea,
- Trichomonas vaginalis,
- Human papillomavirus types 16/18,
- Herpes simplex virus types I and II,
- Cytomegalovirus,
using the polymerase chain reaction method in real time.